What is a CO2 incubator?
The CO2 incubator is a growth environment that simulates the formation of a similar cell/arrangement in the organism inside the incubator.
The incubator requires stability CO2 incubators require stable temperature (37°C), stable CO2 level (5%), stable pH (pH: 7.2-7.4), high relative saturation humidity (95%), for cell/arrangement A device for in vitro cultivation.
Features of carbon dioxide incubators: temperature control, generally only with heating, high temperature sterilization, individual refrigeration, CO2 level control, pH control, humidity control.
Lab line co2 incubator are widely used in cell, arrangement cultivation and cultivation of some special microorganisms.
Carbon dioxide incubator can be used for antigen research and production, cell dynamics research, tissue engineering, carcinogenic or toxicological effects of various physical and chemical factors, collection of stem cells, mammalian cell secretions, hybridoma cell culture to produce antibodies, drugs Screening, in vitro fertilization (IVF) and other research fields.
Due to the increase of carbon dioxide concentration control and the use of micro-manipulators to accurately control the temperature of the incubator, the success rate and efficiency of biological cells, tissues, etc. have been improved.
In a word, the carbon dioxide incubator is a new type of incubator that cannot be replaced by ordinary electric thermostatic incubators.
How to properly operate a CO2 incubator?
1. Do not turn on the power switch before the lab incubator co2 stacked is filled with water, otherwise the heating tube will be damaged.
2. After the incubator has been working continuously for several months, the water in the water tank may be reduced due to volatilization. When the low water level indicator lights up, water needs to be added. First open the overflow pipe, connect the rubber pipe with a funnel to add water from the water injection hole, make the low water level indicator go out, then add water by metering, and then plug the overflow hole.
3. The carbon dioxide incubator can be used as a high-precision constant temperature incubator after the CO2 control system is turned off.
4. Because the CO2 sensor is calibrated under saturated humidity, the humidification tray must be filled with sterile water at all times.
5. When the displayed temperature exceeds the set temperature by 1°C, the over-temperature alarm indicator (OverTemp7) will be on, and a sharp alarm will sound. At this time, the power should be turned off for 30 minutes. If the power (temperature control) switch (Power20) is still overheated, turn off the power and report to the maintenance personnel.
6. When the cylinder pressure is lower than 0.2MPa, the cylinder should be replaced.
7. Minimize the time to open the glass door.
8. If the carbon dioxide incubator is not used for a long time, the moisture in the working room must be removed, and the glass door must be opened for ventilation for more than 24 hours.
9. When cleaning the studio of the carbon dioxide incubator, do not collide with components such as the sensor and the stirring motor fan wheel.
10. When disassembling and assembling the bracket cover in the studio, you must use a special wrench, and do not use excessive force.
11. The water in the incubator must be removed before transporting the incubator. When draining water, tightly cover the rubber tube on the water outlet, so that the mouth of the tube is lower than the instrument, take a light breath, put down the water tube, and the water will flow out by siphon.
12. Before carrying the carbon dioxide incubator, take out the shelf and humidification tray in the work room to prevent collision and damage to the glass door.
13. When transporting the incubator, do not turn it upside down, and do not lift the door to avoid damage to the door.
How to use a CO2 incubator?
1. Before taking items from the incubator, clean your hands (or gloves) with alcohol to shorten the door opening time and reduce the number of door openings as much as possible.
(Note: The air in the incubator is filtered clean air. Opening the incubator for a long time or switching the incubator on and off frequently may cause contamination.)
2. When taking the cells from the incubator, handle it gently, move quickly, and close the incubator door tightly. It is forbidden to look at or move other people's cells or samples without permission. If you have special needs, please contact the person in charge of the laboratory for coordination.
3. Before putting the culture bottle dish into the incubator, disinfect the surface with alcohol, and wait until the alcohol evaporates before putting it in, so as to avoid excessive ethanol vapor remaining in the incubator.
4. The placement of cell culture in the incubator should be neat and orderly to facilitate searching. At the same time, the use efficiency of the incubator should be improved as much as possible. The person in charge can activate or stop the use of the empty incubator depending on the experimental situation to save resources.
5. For cells in ordinary culture, unless there are special needs for the experiment, each bottle/plate of cells only needs to observe the growth state once a day, and the cells shared by more than 2 people can be observed together at an appointment time. (Note: Frequently taking the cells out for observation will easily contaminate the incubator and affect the constant cell growth conditions.)
6. The experimenter should always pay attention to check whether the temperature of the incubator and the amount of CO2 gas match the set value. Pay close attention to the humidification tray inside the incubator, change sterile water regularly and sterilize it.
If there are obvious signs of contamination inside the incubator, such as mold, plaque, mycoplasma, and chlamydia infections, administrators and other users should be notified immediately.
How to sterilize the CO2 incubator?
Many incubators are used for sterilization and disinfection. In this process, there are some precautions that need to be paid more attention.
The volume of the carbon dioxide incubator is a factor that cannot be ignored. If it is too small, it is not enough. If it is too large, it is wasteful and takes up space.
Users are required to have a more accurate understanding of the required incubator volume range before purchasing, and reserve a little more space on this basis to ensure contingencies.
In addition, every user expects the instrument to be convenient and easy to use. The use of the microprocessor control system and various other functional accessories makes the operation and control of the carbon dioxide incubator very simple. Although different microprocessor systems have different names, there is no essential difference in their principles and control effects.
High temperature disinfection is divided into high temperature dry heat disinfection and high temperature moist heat sterilization. Due to the large latent heat of steam and strong penetrating power, high-temperature moist heat can easily denature or solidify proteins, so the sterilization efficiency of this method is higher than that of dry heat sterilization.
Generally speaking, high temperature disinfection is not simply to see the disinfection temperature, but mainly to see whether it is wet heat disinfection.
In addition, from the perspective of use, moist heat disinfection is generally controlled at 90 °C to achieve a very thorough disinfection effect. During the entire disinfection process, all accessories in the incubator do not need to be taken out, and they can all be disinfected.
For dry heat sterilization, in order to achieve better results, the temperature is generally above 100 °C. When purchasing a carbon dioxide incubator, most users will choose an incubator with high-temperature moist heat sterilization.
How do CO2 incubators control pollutants?
Contamination is a major factor in cell culture failure. Manufacturers of CO2 incubators have designed a variety of devices to reduce and prevent contamination. Automatic pollution removal device to effectively prevent pollution.
For example, in view of the fact that the CO2 incubator is sometimes accompanied by mold growth during use, in order to ensure that the incubator is free from contamination and ensure the biological cleanliness of the instrument box, some companies have developed and designed a CO2 incubator with ultraviolet disinfection function.
There is also a company designed HEPA high-efficiency filter to filter the air in the incubator, which can filter and remove 99.97% of particles above 0.3um.
In addition, the automatic high temperature hot air sterilization device can make the temperature in the box reach a high temperature (such as 200 ° C) to kill all polluting microorganisms, even high temperature resistant microorganisms such as spores. These devices are more secure for cell culture.
What is the difference between a CO2 incubator and an anaerobic incubator?
What is an anaerobic incubator?
Anaerobic incubator is a special equipment for bacterial culture operation under anaerobic conditions.
Anaerobic incubator
What is a CO2 incubator?
The carbon dioxide incubator is formed by imitating the growth environment of a similar cell tissue in the living body in the incubator box. The incubator requires a stable temperature (37°C), stable CO2 level (5%), stable pH (pH value) : 7.2-7.4), high relative saturated humidity (95%), a device for in vitro culture of cells and tissues.
CO2 incubators are not anaerobic incubators.
The anaerobic box has no oxygen in the box, and the gas inside is generally composed of a combination of 90% nitrogen + 5% hydrogen + 5% carbon dioxide. The microorganisms cultivated in the anaerobic box are all anaerobic microorganisms;
The carbon dioxide incubator is 5% carbon dioxide + 95% air, and there is a part of oxygen in it.
How to add water to a water jacket co2 incubator?
When the water jacketed co2 incubator is used, it must first add water. Don't think the simple task of adding water is very simple. In fact, the whole process of adding water is also done step by step.
Follow me to introduce you in detail：
Turn on the faucet, slowly add water to the water tank in the water jacketed carbon dioxide incubator, and gradually increase with the water level. When the low water level indicator is off, wait another 3-5 seconds and then stop adding water.
At this time, the water level is between the low and high water levels, the water level lights should not be on, and the water jacket carbon dioxide incubator equipment can be put into operation.
When no water is added to a level higher than the low water level, the low water level indicator light will be on and a buzzer will sound.
If there is too much water, after the high water level indicator is on, the water will overflow from the overflow port on the back of the lab water jacketed co2 incubators.
Therefore, when the high water level indicator is on, pull out the rubber tube of the water outlet on the lower left side and then pull out the water drain plug to drain the water.
2-3 seconds after the high water level indicator goes out, then plug the drain plug tightly.
When the low water level alarms, water should be added in time, otherwise the temperature controller will stop heating.
Why grow cells in a lab co2 incubator?
CO2 is not only a cell metabolite but also a required component of the cell, which is mainly directly related to maintaining the pH of the culture medium.
Most animal cells need a slightly alkaline environment with a pH of around 7.4.
During cell culture, as the amount of carbon dioxide released increases, the medium will become acidic, so NaHCO3 is often added to adjust the pH.
NaHCO3 has a tendency to release carbon dioxide, and the addition of carbon dioxide can inhibit the progress of this reaction.
The CO2 concentration in the incubator must be balanced with the sodium bicarbonate concentration in the medium.
The gas environment of the carbon dioxide incubator is generally 95% air and 5% CO2.
So cell culture needs to be in a humidity co2 incubator.
CO2 incubators will be better developed in the medical field
Nowadays, the application of forma scientific co2 incubator has been very extensive. In medicine, carbon dioxide incubators have played a huge role.
It is understood that compact co2 incubator allow sperm and eggs to freely combine into one or more embryos after collisional encounters.
Nowadays, many families have begun to do IVF. Under normal circumstances, IVF needs to be injected with ovulation-stimulating drugs for more than 10 consecutive days, and then the doctor will remove the eggs from the patient's body and put them into the test tube.
About 1 hour after the egg retrieval begins. Refined.
Then, the staff will mix the best sperm and eggs in the same test tube, let them meet and combine freely.
On the 3rd day after fertilization, when the fertilized egg divides into 8 cells, the embryo transfer operation can be performed, or it can be used for cryopreservation.
After the fertilized egg successfully conceives the embryo, some of it is used for IVF, and the other surplus high-quality embryos available for saving.
In terms of preservation, the liquid nitrogen cryopreservation method is commonly used, which is the biological preservation method with the widest application range and the longest shelf life.
Frozen embryos from the same couple are placed in a cannula and placed in a tank of liquid nitrogen.
This liquid nitrogen temperature is very low, far below the temperature at which the body's metabolism stops.
After this time, all the metabolic activities of the cells have stopped, the chemical effects have also disappeared, and they can be preserved intact.
FAQ
How long can the filter of the CO2 incubator be used?
Half a year to a few years are available.
It would be best if you changed it once a year, as the filter is adsorptive and needs to be changed frequently.
For the small filter, as long as it is not harmful and does not need to replace, it is to play a filtering role, similar to the function of filter paper.
What is the role of a carbon dioxide incubator CO2?
CO2 in the CO2 incubator can play a role in insulation so that the temperature control in the correct range, the principle, and the greenhouse effect are similar.
Why does the carbon dioxide incubator alarm keep ringing?
Lack of circulating water and CO2 depletion can both cause this result.
How to disinfect the CO2 incubator?
CO2 incubator sterilization is a kind of high-temperature sterilization, and the other is ultraviolet sterilization.
The use of higher is ultraviolet sterilization. There are ultraviolet lamps inside.
Carbon dioxide incubator can cultivate anaerobic bacteria?
The answer is no.
Because the gas in the anaerobic chamber is 90% nitrogen + 5% hydrogen + 5% carbon dioxide, there must not be a trace of oxygen.
Because oxygen will destroy an enzyme in the anaerobic organisms, thus killing the anaerobic bacteria.
Carbon dioxide incubator to how to carry out anoxic culture?
The gas environment in the CO₂ incubator can be set and controlled from the panel.
Just set O to 0, set CO2 to 100, or set CO2 and N2 to 100, such as the concentration of CO2, or to maintain 5%, N2 adjusted to 95%.
How much is the power of the CO2 incubator?
The available range is 800-1200w.
How to control CO2 gas concentration in a CO2 incubator?
When a certain pressure of highly concentrated CO2 gas is input, through the flow and time control of regulating valve, needle valve, and solenoid valve, a certain amount of CO2 gas is guaranteed to enter the studio to achieve automatic control of CO2 concentration value in the working chamber.
This value is also displayed through the display circuit for easy observation and understanding.
In order to facilitate the user to monitor and sample the concentration in use, a sampling and monitoring port is installed at the back of the CO2 tank.
The CO2 concentration in the working chamber can be recovered quickly and without overshoot after the CO2 tank is opened and closed because of the double-filling method of fast and slow speed.
During the operation of the CO2 tank, the cylinder is used to replenish the gas in the working chamber to maintain a stable CO2 concentration value.
Why NOKI is the best CO2 incubator manufacturer?
NOKI is from China and has 20 years of experience in the production of laboratory equipment. NOKI has done a very good job in the quality control of CO2 incubators, and can give better prices for CO2 incubator.
NOKI sample room
NOKI also produces other types of incubators, including: electric heating incubators, constant temperature and humidity incubators, light incubators, microbial incubators, light incubators, biochemical incubators, artificial climate incubators, etc.
If you are interested in CO2 incubators, please contact us at seo@zznoki.com!
